A simple method for the determination of reduction potentials in heme proteins
نویسندگان
چکیده
We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em=-115 mV ± 2 mV, pH 7.0) and human CLOCK (Em=-111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.
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عنوان ژورنال:
دوره 588 شماره
صفحات -
تاریخ انتشار 2014